Effective mRNA Inhibition in PANC-1 Cells in Vitro Mediated via an mPEG–SeSe–PEI Delivery System

• Zhang Yuefeng

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

• Yang Bin

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

• Liu Yajie

  MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University

• Qin Wenjie

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

• Li Chao

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

• Wang Lantian

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

• Zheng Wen

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

• Wu Yulian

  Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

書誌事項

タイトル別名

• Effective mRNA Inhibition in PANC-1 Cells <i>in Vitro</i> Mediated <i>via</i> an mPEG–SeSe–PEI Delivery System

抄録

RNA interference (RNAi)-mediated gene therapy is a promising approach to cure various diseases. However, developing an effective, safe, specific RNAi delivery system remains a major challenge. In this study, a novel redox-responsive polyetherimide (PEI)-based nanovector, mPEG–SeSe–PEI, was developed and its efficacy evaluated. We prepared three mPEG–SeSe–PEI vector candidates for small interfering glyceraldehyde-3-phosphate dehydrogenase (siGADPH) and determined their physiochemical properties and transfection efficiency using flow cytometry and PEG_11.6–SeSe–PEI polymer. We investigated the silencing efficacy of GADPH mRNA expression in PANC-1 cells and observed that PEG_11.6–SeSe–PEI/siGADPH (N/P ratio=10) polyplexes possessed the appropriate size and zeta-potential and exhibited excellent in vitro gene silencing effects with the least cytotoxicity in PANC-1 cells. In conclusion, we present PEG_11.6–SeSe–PEI as a potential therapeutic gene delivery system for small interfering RNA (siRNA).

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 39 (5), 680-688, 2016

  公益社団法人 日本薬学会

キーワード

• small interfering RNA (siRNA) delivery system
• RNA interference
• gene therapy
• mPEG–SeSe–polyetherimide (PEI)

詳細情報

• CRID: 1390001204633086976
• NII論文ID: 130005149251
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b15-00645
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 027269938
• PubMed: 26948169
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027269938
• Web Site: https://www.jstage.jst.go.jp/article/bpb/39/5/39_b15-00645/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017000448
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可