Effect of 1.8-Cineole in Dermatophagoides pteronyssinus-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma
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- Lee Hyun-Seung
- Laboratory of Allergy and Clinical Immunology, Dongguk University Ilsan Hospital Medical Research Center
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- Park Da-Eun
- Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center
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- Song Woo-Jung
- Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center
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- Park Heung-Woo
- Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center
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- Kang Hye-Ryun
- Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center
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- Cho Sang-Heon
- Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center
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- Sohn Seong-Wook
- Laboratory of Allergy and Clinical Immunology, Dongguk University Ilsan Hospital Medical Research Center Department of Internal Medicine, Dongguk University Ilsan Hospital
書誌事項
- タイトル別名
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- Effect of <i>1.8-Cineole</i> in <i>Dermatophagoides pteronyssinus</i>-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma
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抄録
1.8-Cineole (eucalyptol) is a phytoncide, a volatile organic compound derived from plants. Phytoncides are known to have an anti-inflammatory effect. However, the effects of 1.8-cineole in house dust mite (HDM)-stimulated bronchial epithelial cells are poorly understood. The objective of this study was to assess the effect of 1.8-cineole in HDM-stimulated bronchial epithelial cells and in the HDM-induced murine asthma model. The purpose of the present study is to evaluate the anti-inflammatory effects and mechanism of 1.8-cineole action in HDM-induced airway inflammation. Human bronchial epithelial cells (HBECs) were cultured with Dermatophagoides pteronyssinus (Der p) and 1.8-cineole. Cytokine protein levels, phosphorylation of protein kinases, and intracellular Toll-like receptor 4 (TLR4) expressions were measured. In the murine model, BALB/C mice were sensitized with Der p and were exposed to Der p via intranasal route during the challenge period. 1.8-Cineole was given by inhalation 6 h before the each challenge. Treatment with 1.8-cineole inhibited the Der p-induced cytokine protein expression, phosphorylation of p38 mitogen-activated protein kinase (MAPK) and Akt and intracellular TLR4 expression in HBECs. In the Der p-induced mouse model, airway hyper-responsiveness (AHR) and the number of eosinophils in bronchoalveolar lavage fluid (BALF) was also significantly reduced by 1.8-cineole treatment. The treatment of 1.8-cineole inhibited the increased production of interleukin (IL)-4, IL-13 and IL-17A in BALF after Der p challenge. These results suggest that 1.8-cineole suppresses Der p-induced IL-8, IL-6 and granulocyte macrophage-colony stimulating factor (GM-CSF) production in HBECs. Finally, we confirmed that 1.8-cineole decreases AHR and eosinophilic airway inflammation in Der p-induced asthma mice.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 39 (6), 946-952, 2016
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679609970304
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- NII論文ID
- 130005155144
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 027325156
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- PubMed
- 27251496
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可