Effectiveness of essential oils of medicinal plants at reducing the amounts of allergen produced by the European house dust mite, Dermatophagoides pteronyssinus (Trouessart)

  • INSUNG Ammorn
    Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Thailand
  • PUMNUAN Jarongsak
    Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Thailand
  • MAHAKITTIKUN Vanna
    Faculty of Medicine, Siriraj Hospital, Mahidol University, Thailand
  • WANGAPAI Teerapong
    Faculty of Medicine, Siriraj Hospital, Mahidol University, Thailand

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  • Effectiveness of essential oils of medicinal plants at reducing the amounts of allergen produced by the European house dust mite, <i>Dermatophagoides pteronyssinus</i> (Trouessart)

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Essential oils obtained from three medicinal plant species, namely, cinnamon (Cinnamomum bejolghota [Buch.-Ham.] Sweet), citronella grass (Cymbopogon nardus Rendle), and clove (Syzygium aromaticum [L.] Merr. & L.M. Perry), were sprayed on allergenic materials (house dust plus spent mite medium produced by the European house dust mite, Dermatophagoides pteronyssinus [Trouessart]) to evaluate the effects of the oils on allergen levels. A 1% solution of each essential oil in 95% ethanol was sprayed on the allergenic materials (which contained about 50μg of allergen per gram of material), and 95% ethanol, water, and untreated allergenic materials were used as controls. The sprayed materials were allowed to stand for 48h, and then allergen levels were analyzed by means of an enzyme-linked immunosorbent assay method. The results showed that the essential oil from C. bejolghota reduced the amount of allergen>99.4%, and the essential oils from C. nardus and S. aromaticum reduced the amounts by 77.1% and 74.0%, respectively. In contrast, the 95% ethanol control reduced the amount of allergen by only 9.8%.

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