The <i>in vivo</i> Pig-a gene mutation assay is applied to study the genotoxicity of procarbazine hydrochloride in Sprague-Dawley rats

DOI Web Site 参考文献19件
  • Pu Jiang
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China Sun Yat-sen University, China
  • Deng Yuanyuan
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China National Centre for Safety Evaluation of Drugs, China
  • Tan Xiaoyan
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China Sun Yat-sen University, China
  • Chen Gaofeng
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China Sun Yat-sen University, China
  • Zhu Cong
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China Sun Yat-sen University, China
  • Qi Naisong
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Wen Hairuo
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Guo Jun
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Wang Xin
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Qiu Yuwen
    Sun Yat-sen University, China
  • Liang Jinqiang
    Sun Yat-sen University, China
  • Fu Xinlu
    Sun Yat-sen University, China
  • Hu Yanping
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Song Jie
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Geng Xingchao
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Wang Chao
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Zhang Lin
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Huang Zhiying
    Sun Yat-sen University, China
  • Li Bo
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China
  • Wang Xue
    National Centre for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, China

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The Pig-a gene is well-known to encode a key enzyme essential in the biosynthesis of glycosylphosphatidylinositol (GPI), which attaches CD molecules (Cluster differentiation), such as CD55 and CD59, to red blood cells (RBCs) membranes. In this study, the blood was marked with the special antigen CD45-PE (Phycoerythrin) to separate the erythrocytes from the leukocytes, then the Pig-a mutant frequency (MF) of RBCs could be investigated without pivotal antigen CD59-FITC (Fluorescein isothiocyanate), and the optimal ENU concentration was determined to be 100 mg/kg/day. The optimal cell number to count and the stability of specimens were determined. At last, the in vivo Pig-a gene mutation assay was utilized to detect the potential genotoxicity of Cis-Dichlorodiammineplatinum (DP), Procarbazine Hydrochloride (PH), and Triptolide (TP). The results indicated that the Pig-a gene mutation obviously occurred in the PH treatment group. In the DP treatment group, an irregular shape with a slightly serrated border in erythrocytes was observed in the blood smear. However, no obvious Pig-a gene mutations were detected in the DP treatment and TP treatment groups under the experimental conditions. In conclusion, this primary in vivo Pig-a gene mutation assay will provide an easy protocol to use in screening potential genotoxic compounds.

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