Transcription of follicle-stimulating hormone subunit genes is modulated by porcine LIM homeobox transcription factors, LHX2 and LHX3
-
- YOSHIDA Saishu
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
-
- KATO Takako
- Institute of Reproduction and Endocrinology, Meiji University, Kanagawa 214-8571, Japan Organization for the Strategic Coordination of Research and Intellectual Property, Meiji University, Kanagawa 214-8571, Japan
-
- NISHIMURA Naoto
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
-
- KANNO Naoko
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
-
- CHEN Mo
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
-
- UEHARU Hiroki
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
-
- NISHIHARA Hiroto
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
-
- KATO Yukio
- Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan Institute of Reproduction and Endocrinology, Meiji University, Kanagawa 214-8571, Japan Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, Meiji University, Kanagawa 214-8571, Japan
この論文をさがす
抄録
The LIM-homeobox transcription factors LHX2 and LHX3s (LHX3a and LHX3b) are thought to be involved in regulating the pituitary glycoprotein hormone subunit genes Cga and Fshβ. These two factors show considerable differences in their amino acid sequences for DNA binding and protein-protein interactions and in their vital function in pituitary development. Hence, we compared the DNA binding properties and transcriptional activities of Cga and Fshβ between LHX2 and LHX3s. A gel mobility shift assay for approximately 1.1 kb upstream of Cga and 2.0 kb upstream of Fshβ varied in binding profiles between LHX2 and LHX3s. DNase I footprinting revealed DNA binding sites in 8 regions of the Cga promoter for LHX2 and LHX3s with small differences in the binding range and strength. In the Fshβ promoter, 14 binding sites were identified for LHX2 and LHX3, respectively. There were alternative binding sites to either gene in addition to similar differences observed in the Cga promoter. The transcriptional activities of LHX2 and LHX3s according to a reporter assay showed cell-type dependent activity with repression in the pituitary gonadotrope lineage LβT2 cells and stimulation in Chinese hamster ovary lineage CHO cells. Reactivity of LHX2 and LHX3s was observed in all regions, and differences were observed in the 5'-upstream region of Fshβ. However, immunohistochemistry showed that LHX2 resides in a small number of gonadotropes in contrast to LHX3. Thus, LHX3 mainly controls Cga and Fshβ expression.
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 62 (3), 241-248, 2016
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206337654656
-
- NII論文ID
- 130005157181
-
- NII書誌ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 027429377
-
- PubMed
- 26853788
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可