Gallic Acid, the Active Ingredient of Terminalia bellirica, Enhances Adipocyte Differentiation and Adiponectin Secretion

• Makihara Hiroko

  Department of Molecular Pharmacology and Neurobiology, Graduate School of Medicine, Yokohama City University Biological Science and Nursing, Graduate School of Medicine, Yokohama City University

• Koike Yuka

  Division of Pharmacognosy and Phytochemistry, Department of Medicinal Chemistry, Showa University

• Ohta Masatomi

  Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University

• Horiguchi-Babamoto Emi

  Faculty of Pharmacy, Musashino University

• Tsubata Masahito

  Research and Development Division, Toyo Shinyaku Co., Ltd.

• Kinoshita Kaoru

  Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University

• Akase Tomoko

  Biological Science and Nursing, Graduate School of Medicine, Yokohama City University

• Goshima Yoshio

  Department of Molecular Pharmacology and Neurobiology, Graduate School of Medicine, Yokohama City University

• Aburada Masaki

  Faculty of Pharmacy, Musashino University

• Shimada Tsutomu

  Department of Hospital Pharmacy, University Hospital, Kanazawa University

書誌事項

タイトル別名

• Gallic Acid, the Active Ingredient of <i>Terminalia bellirica</i>, Enhances Adipocyte Differentiation and Adiponectin Secretion

抄録

Visceral obesity induces the onset of metabolic disorders such as insulin resistance and diabetes mellitus. Adipose tissue is considered as a potential pharmacological target for treating metabolic disorders. The fruit of Terminalia bellirica is extensively used in Ayurvedic medicine to treat patients with diseases such as diabetes mellitus. We previously investigated the effects of a hot water extract of T. bellirica fruit (TB) on obesity and insulin resistance in spontaneously obese type 2 diabetic mice. To determine the active ingredients of TB and their molecular mechanisms, we focused on adipocyte differentiation using mouse 3T3-L1 cells, which are widely used to study adipocyte physiology. We show here that TB enhanced the differentiation of 3T3-L1 cells to mature adipocytes and that one of the active main components was identified as gallic acid. Gallic acid (10–30 µM) enhanced the expression and secretion of adiponectin via adipocyte differentiation and also that of fatty acid binding protein-4, which is the target of peroxisome proliferator-activated receptor gamma (PPARγ), although it does not alter the expression of the upstream genes PPARγ and CCAAT enhancer binding protein alpha. In the PPARγ ligand assay, the binding of gallic acid to PPARγ was undetectable. These findings indicate that gallic acid mediates the therapeutic effects of TB on metabolic disorders by regulating adipocyte differentiation. Therefore, TB shows promise as a candidate for preventing and treating patients with metabolic syndrome.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 39 (7), 1137-1143, 2016

  公益社団法人 日本薬学会

キーワード

• <i>Terminalia bellirica</i>
• gallic acid
• adipocyte differentiation
• adiponectin
• metabolic-disorder therapy

詳細情報

• CRID: 1390282679608249472
• NII論文ID: 130005160767
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b16-00064
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 027450886
• PubMed: 27374289
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027450886
• Web Site: https://www.jstage.jst.go.jp/article/bpb/39/7/39_b16-00064/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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