Ultrastructural localization of endogenous exchange factor for ARF6 in adrenocortical cells In situ of mice
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- Chomphoo Surang
- Electron Microscopy Laboratory, Department of Anatomy, Faculty of Medicine, Khon Kaen University Neuroscience Research and Development Group, Khon Kaen University
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- Mothong Wilaiwan
- Electron Microscopy Laboratory, Department of Anatomy, Faculty of Medicine, Khon Kaen University
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- Sawatpanich Tarinee
- Electron Microscopy Laboratory, Department of Anatomy, Faculty of Medicine, Khon Kaen University
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- Kanla Pipatphong
- Electron Microscopy Laboratory, Department of Anatomy, Faculty of Medicine, Khon Kaen University
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- Sakagami Hiroyuki
- Department of Anatomy, School of Medicine, Kitasato University
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- Kondo Hisatake
- Electron Microscopy Laboratory, Department of Anatomy, Faculty of Medicine, Khon Kaen University
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- Hipkaeo Wiphawi
- Electron Microscopy Laboratory, Department of Anatomy, Faculty of Medicine, Khon Kaen University
書誌事項
- タイトル別名
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- Ultrastructural Localization of Endogenous Exchange Factor for ARF6 in Adrenocortical Cells <i>In Situ</i> of Mice
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抄録
EFA6 (exchange factor for ARF6) activates Arf6 (ADP ribosylation factor 6) by exchanging ADP to ATP, and the resulting activated form of Arf6 is involved in the membrane dynamics and actin re-organization of cells. The present study was attempted to localize EFA6 type D (EFA6D) in mouse adrenocortical cells in situ whose steroid hormone secretion is generally considered not to depend on the vesicle-involved regulatory mechanism. In immunoblotting, an immunoreactive band with the same size as brain EFA6D was detected in homogenates of adrenal cortical tissues almost free of adrenal capsules and medulla. In immuno-light microscopy, EFA6D-immunoreactivity was positive in adrenocortical cells and it was often distinct along the plasmalemma, especially along portions of the cell columns facing the interstitium. In immuno-electron microscopy, the gold-labeling was more dense in the peripheral intracellular domains than the central domain of the immunopositive cells. The labeling was deposited on the plasma membranes in a discontinuous pattern and in cytoplasmic domains rich in filaments. It was also associated with some, but not all, of pleiomorphic vesicles and coated pits/vesicles. No labeling was seen in association with lipid droplets or smooth endoplasmic reticulum. The present finding is in support of the importance of EFA6D for activation of Arf6 in adrenocortical cells.
収録刊行物
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- Acta Histochemica et Cytochemica
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Acta Histochemica et Cytochemica 49 (3), 83-87, 2016
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詳細情報 詳細情報について
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- CRID
- 1390001204862112384
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- NII論文ID
- 130005161005
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- ISSN
- 13475800
- 00445991
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可