Pathogenicity of two Candidatus Xenohaliotis californiensis genetic variants against three abalone species (the genus Haliotis)

• Nishioka Toyohiro

  Kamiura Laboratory, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Kamaishi Takashi

  Fisheries Agency, Ministry of Agriculture, Forestry and Fisheries

• Kurita Jun

  Tamaki Laboratory, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Mekata Tohru

  National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Kiryu Ikunari

  Tamaki Laboratory, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Yuasa Kei

  National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Shimahara Yoshiko

  Kamiura Laboratory, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Hyoudou Junko

  Agriculture Forestry and Fishery Division of Bureau of Industrial and Labor Affairs, Tokyo Metropolitan Government

• Ryu Takehiro

  Tokyo Metropolitan Oshima Island Branch Office

• Takase Tomohiro

  Tokyo Metropolitan Islands Area Research and Development Center for Agriculture, Forestry and Fisheries

• Uchimura Yuushi

  Ehime Fisheries Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries

• Ototake Mitsuru

  National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

• Oseko Norihisa

  Hokkaido National Fisheries Research Institute, Japan Fisheries Research and Education Agency

書誌事項

タイトル別名

• Pathogenicity of two <i>Candidatus</i> Xenohaliotis californiensis genetic variants against three abalone species (the genus <i>Haliotis</i>)

抄録

Candidatus Xenohaliotis californiensis, a Rickettsia-like organism (RLO) that causes ‘withering syndrome’ (WS) in abalone, has been recently detected in Japan. We analyzed partial nucleotide sequence (113 bp) of 16S rRNA of WS-RLOs (n = 335) from Japanese black abalone Haliotis discus discus, Ezo abalone H. discus hannai, giant abalone H. gigantea, tokobushi abalone H. diversicolor aquatilis and fukutokobushi abalone H. diversicolor diversicolor. All the sequences from Japanese black, Ezo and giant abalone were identical, but different from those from tokobushi and fukutokobushi at one nucleotide position. We also conducted cohabitation challenge to determine whether the WS-RLO in fukutokobushi infects Japanese black and giant abalone or the agent in Japanese black abalone infects fukutokobushi. Twenty fukutokobushi naturally infected with WS-RLO were cohabited with 10 healthy individuals of each of Japanese black, giant, and fukutokobushi abalone for a total of 84 days. At the end of the experiment, surviving fukutokobushi abalone were positive in PCR test for WS-RLO, but negative for that of Japanese black and Ezo abalone. In the reverse combination experiment, in which naturally infected Japanese black abalone were cohabited with these three species, WS-RLO transmissions were found in Japanese black and Ezo, but not in fukutokobushi abalone. These results suggest that two genetic variants of WS-RLO have a different host specificity.

収録刊行物

• 魚病研究

  魚病研究 51 (2), 54-59, 2016

  日本魚病学会

キーワード

• withering syndrome
• <i>Candidatus</i> Xenohaliotis californiensis
• pathogenicity
• <i>Haliotis diversicolor diversicolor</i>
• <i>Haliotis discus discus</i>
• 16S rRNA
• abalone

詳細情報

• CRID: 1390282681436903040
• NII論文ID: 130005163045
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.51.54
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 027506037
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027506037
• Web Site: https://www.jstage.jst.go.jp/article/jsfp/51/2/51_54/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可