Determination of the Molar Extinction Coefficients of the B800 and B850 Absorption Bands in Light-harvesting Complexes 2 Derived from Three Purple Photosynthetic Bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides, and Phaeospirillum molischianum by Extraction of Bacteriochlorophyll a

• SAGA Yoshitaka

  Department of Chemistry, Faculty of Science and Engineering, Kinki University PRESTO, Japan Science and Technology Agency

• HIROTA Keiya

  Department of Chemistry, Faculty of Science and Engineering, Kinki University

書誌事項

タイトル別名

• Determination of the Molar Extinction Coefficients of the B800 and B850 Absorption Bands in Light-harvesting Complexes 2 Derived from Three Purple Photosynthetic Bacteria <i>Rhodoblastus acidophilus</i>, <i>Rhodobacter sphaeroides</i>, and <i>Phaeospirillum molischianum</i> by Extraction of Bacteriochlorophyll <i>a</i>

抄録

The molar extinction coefficients of light-harvesting complex 2 (LH2) have been ambiguous in spite of its fame and wide utilization. Herein we determine the molar extinction coefficients of the LH2 proteins derived from the three purple photosynthetic bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides and Phaeospirillum molischianum at 298 K by direct extraction of bacteriochlorophyll (BChl) a from the lyophilized proteins, followed by estimation of BChl a amounts from their electronic absorption spectra.

収録刊行物

• Analytical Sciences

  Analytical Sciences 32 (7), 801-804, 2016

  社団法人 日本分析化学会

キーワード

• Bacteriochlorophyll
• B800
• B850
• molar extinction coefficient
• photosynthetic protein

詳細情報

• CRID: 1390282679237665792
• NII論文ID: 130005163080
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.32.801
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 027497186
• PubMed: 27396664
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027497186
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.801.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.801/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/7/32_801/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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