Is Bone Mineral Density Associated with Estrogen Receptor a in Chinese Men? A Pilot Study

• Lin Yu

  Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen University

• Zhang Yiyuan

  Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen University

• Huang Yunmei

  Fujian Academy of Integrative Medicine (Fujian University of Traditional Chinese Medicine)

• Wang Wulian

  Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen University

• Feng Eryou

  Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen University

• Lin Yanping

  Fujian Academy of Integrative Medicine (Fujian University of Traditional Chinese Medicine)

抄録

This study aimed to assess the associations of telomerase reverse transcriptase (TERT) and estrogen receptor-α (ERα) levels with bone mineral density in Chinese men and women. Cancellous bone samples were obtained from femoral neck fracture and osteoarthritis patients. BMD was evaluated by X-ray absorptiometry. Serum levels of BMD markers, including osteocalcin (BGP), tartrate-resistant acid phosphatase 5b (TRACP5b), estradiol (E2) and testosterone (T), were measured by ELISA. Real-time PCR and Western blot were used to assess hTERT, ERα, and human protection of telomeres 1 (hPot1) mRNA and protein levels, respectively, in bone specimens. Age, E2, T, TRACP5b, BGP, TNF-α, NF-kB and Pot1 mRNA levels, as well as TERT and ERα mRNA and protein levels were all correlated with BMD (FN) and BMD (AP) as single variables, except for sex in the case of BMD (FN). Multiple regression analysis revealed sex, age, E2, T, and TERT protein to be significantly associated with BMD (AP); similar findings were obtained with BMD (FN), except for sex. Interestingly, sex, TERT mRNA, and ERα protein showed significant correlations with both BMD (FN) and BMD (AP) (all P<0.01) after collinearity diagnostics. Importantly, gender differences were observed for BMD (AP) and BMD (FN) correlations with TERT and ERα protein and gene levels. These findings indicate that TERT and ERα are associated with BMD in Chinese individuals, with differences observed in these associations between men and women.

収録刊行物

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 25 (3), 288-294, 2016

  硬組織再生生物学会

キーワード

• Bone mineral density
• Estrogen receptor
• Gender differences
• TERT

詳細情報

• CRID: 1390282679434585344
• NII論文ID: 130005163150
• NII書誌ID: AA11074332
• DOI: 10.2485/jhtb.25.288
• ISSN: 1880828X
• ISSN: 13417649
• NDL書誌ID: 027531906
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027531906
• Web Site: https://www.jstage.jst.go.jp/article/jhtb/25/3/25_288/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可