miRNAとmRNAの統合解析によるがん細胞の低酸素適応のメカニズム解析

• 大木 拓也

• 梅津 知宏

• 大屋敷 純子

• 福岡 豊

書誌事項

タイトル別名

• Investigation of mechanism for hypoxia tolerance in cancer cells by integrative analysis of miRNA and mRNA expressions

抄録

The purpose of this study is to investigate mechanisms for acquisition of hypoxic tolerance in cancer cells. We cultured RPMI 8226 cell line, which was derived from the peripheral blood of a multiple myeloid patient, in the following three conditions: normoxic, acute and chronic hypoxic conditions. Then, expression levels of the microRNAs and the mRNAs were measured in each condition. The microRNAs whose expression levels changed more than double and the mRNAs whose expression levels changed significantly were detected by pair-wised comparisons. Because a microRNA controls expression levels of mRNAs, we investigated the expression data in the following steps. First, we detected the target mRNAs of a microRNA. From these, we extracted the significantly changed mRNAs. The functions of the target mRNAs were investigated using the DAVID bioinformatics tools. These steps were repeated for all microRNAs. Results of the integrative analysis indicated that negative cell proliferation and angiogenesis were significantly enriched. It is suggested that these functions are related to cancer cells' acquisition of hypoxic tolerance.

収録刊行物

• 生体医工学

  生体医工学 53 (Supplement), S236_03-S236_03, 2015

  公益社団法人 日本生体医工学会

詳細情報

• CRID: 1390001205268201984
• NII論文ID: 130005163940
• DOI: 10.11239/jsmbe.53.s236_03
• ISSN: 18814379
• ISSN: 1347443X
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可