Eosinophil chemotaxis assay in nasal polyps by using a novel optical device EZ-TAXIScan: Role of CC-chemokine receptor 3

• Hidekazu Saito

  Department of Otorhinolaryngology and Head and Neck Surgery, Akita University Graduate School of Medicine

• Honda Kohei

  Department of Otorhinolaryngology and Head and Neck Surgery, Akita University Graduate School of Medicine

• Asaka Chikara

  Department of Otorhinolaryngology and Head and Neck Surgery, Akita University Graduate School of Medicine

• Ueki Shigeharu

  Department of General Internal Medicine and Clinical Laboratory Medicine, Akita University Graduate School of Medicine

• shikawa Kazuo

  Department of Otorhinolaryngology and Head and Neck Surgery, Akita University Graduate School of Medicine

抄録

Background: The chemokine receptor, CC-chemokine receptor 3 (CCR3), and its major ligands, eotaxin, RANTES, and MCP-4, are involved in eosinophil chemotaxis. It is thought that CCR3 plays an important role in the recruitment and activation of eosinophils in nasal polyposis. We examined nasal polyp extract-induced eosinophil chemotaxis and the effect of a CCR3 antagonist using EZ-TAXIScan, a novel real-time chemotaxis assay device. Methods: Nasal polyps were obtained from chronic rhinosinusitis (CRS) patients during surgery. The polyps were homogenized and eotaxin levels in the extracts were measured. Eosinophils were purified from human peripheral blood by the CD16 negative selection method. Nasal polyp extract-induced eosinophil chemotaxis, with or without CCR3 antagonist, was assessed by EZ-TAXIScan. Results: There was a significant positive correlation between the eosinophil counts in nasal polyp and eotaxin levels in the nasal polyp extracts. Using EZ-TAXIScan, eosinophil chemotactic responses were observed following stimulation with nasal polyp extracts. There was a significant positive correlation between the chemotactic index toward the nasal polyp extracts and their eotaxin levels. Nasal polyp extract-induced chemotaxis was completely inhibited by CCR3 antagonist but not by chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) antagonist which inhibited PGD2- induced eosinophil chemotaxis. Conclusions: The CCR3 pathway may play an important role in the pathogenesis of eosinophil recruit ment in nasal polyps through selective eosinophil chemotaxis.

収録刊行物

• Allergology International

  Allergology International 65 (3), 280-285, 2016

  一般社団法人日本アレルギー学会

キーワード

• Asthma; Chronic sinusitis; Eosinophils; Eotaxin; Nasal polyps

詳細情報

• CRID: 1390001204634279552
• NII論文ID: 130005165863
• DOI: 10.1016/j.alit.2016.01.001
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016000022?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016000022?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2017189671
• 本文言語コード: en
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