Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

DOI Web Site Web Site 被引用文献2件 参考文献30件
  • Murayama Kazuyoshi
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University
  • Kawakami Miyuki
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University Division of Cell Regeneration and Transplantation, Advanced Research Center, School of Life Dentistry at Niigata, The Nippon Dental University
  • Tanaka Akira
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University Division of Cell Regeneration and Transplantation, Advanced Research Center, School of Life Dentistry at Niigata, The Nippon Dental University

この論文をさがす

抄録

<p>To examine the pathology of salivary glands that have undergone atrophy or hypofunction due to old age or disease, the duct ligation model is used. This model has been used in studies on the course of glandular parenchyma atrophy and the potential for repair and regeneration. However, the period of ligation was short in most of these studies, and none have examined long-term progress. Therefore, we investigated long-term ligation of the submandibular gland in mice and examined the chronic changes in atrophied salivary glands. The ligation periods were 1, 2 and 3 months, and atrophied salivary glands were resected after each period. Resected glands were examined by histology, immunohistochemistry, RT-PCR, and transmission electron microscopy. Histologically, disappearance of acinar cells and increases in duct-like structures were observed over time in atrophied salivary glands, resulting from long-term submandibular gland main excretory duct ligation. Acinar cell markers (α-amylase, aquaporin 5) showed marked weakness in expression after ligation, but expression was still observed after 3 months of ligation. Stem cell markers (c-kit, Sca-1) showed greater expression at 1 month of ligation, compared with controls, but expression subsequently decreased with time. Expression of the precursor cell marker cytokeratin 5 was retained throughout long-term ligation. Atrophied salivary gland tissue resulting from long-term ligation showed increases in specific duct-like structures over time, and the expression of stem cell markers and progenitor cell markers in the area of these structures suggests that the repair capability remained intact.</p>

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (30)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ