Multiple free-radical scavenging (MULTIS) capacity in cattle serum

• Sueishi Yoshimi

  Department of Chemistry, Faculty of Science, Okayama University

• Kamogawa Erisa

  Department of Chemistry, Faculty of Science, Okayama University

• Kimura Anna

  Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki

• Kitahara Go

  Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki

• Satoh Hiroyuki

  Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki

• Asanuma Taketoshi

  Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki

• Oowada Shigeru

  Dialysis Center, Asao Clinic

抄録

Multiple free-radical scavenging (MULTIS) activity in cattle and human sera was evaluated with electron spin resonance spectroscopy. Scavenging rates against six active species, namely hydroxyl radical, superoxide anion, alkoxyl radical, alkylperoxyl radical, methyl radical, and singlet oxygen were quantified. The difference in the electron spin resonance signal intensity in the presence and absence of the serum was converted into the scavenging rates. Comparative MULTIS measurements were made in sera from eight beef cattle, three fetal calves and fifteen healthy human volunteers. Further, we determined the MULTIS value of albumin, the most abundant component in serum. MULTIS values in cattle sera indicated higher scavenging activity against most free radical species tested than human sera. In particular, cattle serum scavenging activities against superoxide and methyl radical were higher than human serum by 2.6 and 3.7 fold, respectively. In cattle serum, albumin appears to play a dominant role in MULTIS activity, but in human serum that is not the case. Previous data indicated that the abundance of uric acid in bovine blood is nearly 80% less than humans; however, this difference does not explain the deviation in MULTIS profile.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 60 (1), 76-80, 2017

  一般社団法人　日本酸化ストレス学会

キーワード

• cattle serum
• multiple radical scavenging capacity
• MULTIS
• antioxidants
• spin trapping

詳細情報

• CRID: 1390001204673737472
• NII論文ID: 130005188925
• DOI: 10.3164/jcbn.15-142
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://www.jstage.jst.go.jp/article/jcbn/60/1/60_15-142/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017307903
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可