味覚神経伝達を担う新規形質膜ATP放出イオンチャネルCALHM 1の 同定と上皮細胞イオンチャネルの膜局在の数理モデル解析

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  • 樽野 陽幸
    京都府立医科大学大学院医学研究科　細胞生理学

書誌事項

タイトル別名
  • Discovery of New ATP Release Ion Channel, CALHM1, and Mathematical Modeling of ENaC Recycling
  • ミカク シンケイ デンタツ オ ニナウ シンキ ケイシツマク ATP ホウシュツ イオンチャネル CALHM1 ノ ドウテイ ト ジョウヒ サイボウ イオンチャネル ノ マクキョクザイ ノ スウリ モデル カイセキ

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抄録

Epithelial sodium channel (ENaC) plays pivotal roles in maintaining body fluid content and blood pressure via mediating transepithelial Na+ transport in the distal nephron of the kidney. The total Na+ transport activity of the distal nephron epithelium depends on the number of ENaCs located on the apical membrane, which is determined by the recycling rates of insersion and endocytosis. My colleagues and I established a novel method to estimate the total number of recycled ENaCs and the ENaC recycling rates by using a specific and irreversible inhibitor of ENaC for functional labelling of the channels in recycling. Using this method, we discovered a novel negative feedback regulation of ENaC recycling by the total number of recycled channels. On the other study, we identified calcium homeostasis modulator 1 (CALHM1) channel as a novel ATP permeable, voltage–gated ion channel by observing ATP release from CALHM1–expressing cells into the extracellular milieu in response to maneuvers that can activate CALHM1 channels. Furthermore, we found that CALHM1 is essentially required for reception of sweet, bitter and umami tastes by mediating the purinergic neurotransmission of tastes from taste bud cells to the gustatory nerve terminals.

収録刊行物

  • 膜

    膜 40 (5), 291-295, 2015

    日本膜学会

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