Development of a lipopolysaccharide (LPS)-supplemented adjuvant and its effects on cell-mediated and humoral immune responses in male rats immunized against sperm

• NOGUCHI Junko

  Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki 305-8602, Japan

• WATANABE Shinya

  Institute of Livestock and Grassland Science, NARO, Ibaraki 305-0901, Japan

• NGUYEN Thanh Q. Dang

  Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki 305-8602, Japan

• KIKUCHI Kazuhiro

  Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki 305-8602, Japan

• KANEKO Hiroyuki

  Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki 305-8602, Japan

抄録

Supplementation with lipopolysaccharide (LPS) from non-pathogenic Escherichia coli was found to enhance the adjuvant effects of a veterinary vaccine adjuvant (ISA 71VG^®). Sperm immunization using 71VG as an adjuvant in the immature period induced infertility in 25% of male rats, whereas this increased to 62.5% after immunization with 71VG + LPS or Freund’s complete adjuvant (FCA). Mean testicular weight of non-sterile males in the 71VG + LPS group was significantly lower than that in the 71VG or FCA group. Histological examination of testicular tissue from sterile males demonstrated severe impairment of spermatogenesis due to experimental autoimmune orchitis, a cell-mediated autoimmune condition. The serum anti-sperm titer was elevated in the three sperm-immunized groups relative to male rats treated with adjuvant alone, but the titer was higher in the 71VG + LPS and FCA groups than in the 71VG group. We consider that this LPS-supplemented adjuvant stimulates both humoral and cell-mediated immune responses to an extent comparable to FCA.

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 63 (1), 111-115, 2017

  公益社団法人 日本繁殖生物学会

キーワード

• Adjuvant
• Autoimmune orchitis
• Lipopolysaccharide (LPS)
• Rat
• Sperm immunization

詳細情報

• CRID: 1390282681314857472
• NII論文ID: 130005332262
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.2016-144
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 027985651
• PubMed: 27890874
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027985651
• Web Site: https://www.jstage.jst.go.jp/article/jrd/63/1/63_2016-144/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017230674
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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