様々な遺伝子改変マウスを用いた睡眠覚醒制御に関わる視床下部神経のin vitroおよびin vivo神経回路機能解析

DOI
  • 山中 章弘
    筑波大院・基礎医・薬理学 ERATO 柳沢オーファン受容体プロジェクト 科学技術振興機構

書誌事項

タイトル別名
  • In vitro and in vivo analyses of the neuronal network involved in the regulation of sleep/wakefulness using transgenic mice

抄録

Orexin A and B are a pair of neuropeptides implicated in the regulation of sleep/wakefulness and energy homeostasis. The regulatory mechanism of orexin neurons is poorly understood since the small number of orexin neurons is sparsely distributed in the lateral hypothalamus. We made the following transgenic (Tg) mice to study the physiological role in the regulation of sleep/wakefulness.Orexin/cameleon Tg mice, in which orexin neurons specifically express calcium sensing protein, were used for calcium imaging to screen what kind of neurotransmitter affects the activity of orexin neurons.Orexin/EGFP Tg mice, in which orexin neurons express EGFP, were used for electrophysiological studies to reveal intracellular mechanisms involved in the activation or inhibition response. Orexin/GFP::TTC Tg mice, in which orexin neurons express a retrograde tracer, were used for immunohistochemical studies to reveal which neurons directly innervate orexin neurons. These studies using Tg mice revealed how orexin neurons are regulated by afferent neurons. [J Physiol Sci. 2006;56 Suppl:S37]

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詳細情報 詳細情報について

  • CRID
    1390001205729083008
  • NII論文ID
    130005448079
  • DOI
    10.14849/psjproc.2006.0_037_3
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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