アゴニスト誘発性血管平滑筋収縮には1型イノシトール3リン酸受容体サブタイプが主に関与する

• 中村 健

  順天堂大・医・第二生理 科学技術振興機構・国際・カルシウム振動プロジェクト

書誌事項

タイトル別名

• Primary role of inositol 1,4,5-trisphosphate receptor type 1 in agonist-induced aortic contraction in mice

抄録

Contraction of vascular smooth muscles is under the regulation of sympathetic activity and vasoactive hormones. It is known that release of Ca²⁺ from inositol 1,4,5-trisphosphate (IP₃)-sensitive stores causes the initial phase of agonist-induced vasocontraction. In the present study, phenylephrine (PE)-induced contraction was measured in thoracic aortas isolated from the wild-type (WT) and IP₃ receptor type 1 knockout (IP3R1-KO) mice, in order to specify the IP₃ receptor subtype responsible for the agonist-induced contraction. PE (10⁻⁸–10⁻⁶ M)-induced aortic contraction in the IP3R1-KO mice was greatly diminished, compared to that in WT mice, and lacked the steep contraction which was invariably seen in WT aortas immediately after PE application at 10⁻⁶ M. But, high K⁺-induced contraction was indistinguishable between WT and IP3R1-KO aortas. Immunoblotting analysis demonstrated the presence of three IP₃ receptor subtypes (IP3R1, IP3R2 and IP3R3) in WT mouse thoracic aorta; however, abundance of each subtype was in the order of IP3R1 > IP3R3 >> IP3R2. These results indicate that IP3R1 constitutes the Ca²⁺ release channels critical to vasocontraction regulated by sympathetic activity and vasoactive hormones. [J Physiol Sci. 2006;56 Suppl:S42]

収録刊行物

• 日本生理学会大会発表要旨集

  日本生理学会大会発表要旨集 2006 (0), 042-042, 2006

  一般社団法人　日本生理学会

キーワード

• vascular smooth muscle
• IP3 receptor
• contraction

詳細情報

• CRID: 1390001205728932224
• NII論文ID: 130005448130
• DOI: 10.14849/psjproc.2006.0_042_3
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可