Selective Absorption of Dietary Sphingoid Bases from the Intestine via Efflux by P-Glycoprotein in Rats

• FUJII Aoi

  Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• MANABE Yuki

  Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• AIDA Kazuhiko

  Innovation Center, Nippon Flour Mills Co., Ltd.

• TSUDUKI Tsuyoshi

  Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University

• HIRATA Takashi

  Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University Department of Rehabilitation, Shijonawategakuen University

• SUGAWARA Tatsuya

  Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

抄録

Various physiological functions of dietary sphingolipids, such as preventing inflammation and improving the skin barrier function, have been recently demonstrated. The sphingolipid most commonly used as a foodstuff is glucosylceramide from plant sources, which is composed of sphingoid bases that are distinctive from those found in mammals. Although the structure of sphingoid bases in higher plants is more complicated than the structure of those in mammals, the fate of dietary sphingolipids of plant origin is still not understood. In the present study, we investigated the absorption of 4,8-sphingadienine that originated from maize glucosylceramide in the rat intestine by using a lipid absorption assay of lymph collected from the thoracic duct. The cumulative recovery of 4,8-sphingadienine in the lymph was lower than that of sphingosine. Verapamil, a P-glycoprotein inhibitor, significantly increased the absorption of 4,8-sphingadienine but did not affect the absorption of sphingosine. Plant-derived sphingoid bases were detected in the ceramide fraction of lymph fluid by using liquid chromatography-mass spectrometry analysis. These results indicate that 4,8-sphingadienine that originates from the glucosylceramide of higher plants is poorly absorbed in the intestine because of efflux by P-glycoprotein and can be incorporated into a ceramide moiety, at least in part, in intestinal endothelial cells.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ 63 (1), 44-50, 2017

  一般財団法人 学会誌刊行センター

キーワード

• sphingolipids
• sphingoid bases
• sphingadienine
• intestinal absorption
• P-glycoprotein

詳細情報

• CRID: 1390001206324767616
• NII論文ID: 130005492529
• NII書誌ID: AA00703822
• DOI: 10.3177/jnsv.63.44
• ISSN: 18817742
• ISSN: 03014800
• NDL書誌ID: 028009232
• PubMed: 28367925
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028009232
• Web Site: https://www.jstage.jst.go.jp/article/jnsv/63/1/63_44/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017327001
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可