Complete Genome Sequence of Pseudomonas chlororaphis subsp. aurantiaca Reveals a Triplicate Quorum-Sensing Mechanism for Regulation of Phenazine Production

• Morohoshi Tomohiro

  Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University

• Yamaguchi Takahito

  Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University

• Xie Xiaonan

  Center for Bioscience Research and Education, Utsunomiya University

• Wang Wen-zhao

  Key Laboratory for Eco-Efficient Polysilicate Materials, School of Environment and Energy, Peking University Shenzhen Graduate School

• Takeuchi Kasumi

  Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization

• Someya Nobutaka

  Institute of Vegetable and Floriculture Sciences, National Agriculture and Food Research Organization

書誌事項

タイトル別名

• Complete Genome Sequence of <i>Pseudomonas chlororaphis</i> subsp. <i>aurantiaca</i> Reveals a Triplicate Quorum-Sensing Mechanism for Regulation of Phenazine Production
• Complete genome sequence of Pseudomonas chlororaphis subsp. aurantiaca reveals a triplicate quorum-sensing mechanism for regulation of phenazine paroduction

抄録

Pseudomonas chlororaphis subsp. aurantiaca StFRB508 regulates phenazine production through N-acyl-l-homoserine lactone (AHL)-mediated quorum sensing. Two sets of AHL-synthase and AHL-receptor genes, phzI/phzR and aurI/aurR, have been identified from the incomplete draft genome of StFRB508. In the present study, the complete genome of StFRB508, comprising a single chromosome of 6,997,933 bp, was sequenced. The complete genome sequence revealed the presence of a third quorum-sensing gene set, designated as csaI/csaR. An LC-MS/MS analysis revealed that StFRB508 produced six types of AHLs, with the most important AHL being N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-OH-C6-HSL). PhzI mainly catalyzed the biosynthesis of 3-OH-C6-HSL, while AurI and CsaI catalyzed that of N-hexanoyl-l-homoserine lactone and N-(3-oxohexanoyl)-l-homoserine lactone, respectively. A mutation in phzI decreased phenazine production, whereas that in aurI or csaI did not. A phzI aurI csaI triple mutant (508ΔPACI) did not produce phenazine. Phenazine production by 508ΔPACI was stimulated by exogenous AHLs and 3-OH-C6-HSL exerted the strongest effects on phenazine production at the lowest concentration tested (0.1 μM). The plant protection efficacy of 508ΔPACI against an oomycete pathogen was lower than that of wild-type StFRB508. These results demonstrate that the triplicate quorum-sensing system plays an important role in phenazine production by and the biocontrol activity of StFRB508.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 32 (1), 47-53, 2017

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• <i>Pseudomonas chlororaphis</i>
• quorum sensing
• acylhomoserine lactone
• phenazine
• biocontrol

詳細情報

• CRID: 1390001204346370048
• NII論文ID: 130005519590
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me16162
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 028095444
• PubMed: 28239068
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028095444
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/32/1/32_ME16162/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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