5'-non-transcribed flanking region and 5'-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in Arabidopsis roots

• Maruyama-Nakashita Akiko

  Graduate School of Agricultural Science, Kyushu University RIKEN Plant Science Center

• Suyama Akiko

  Graduate School of Agricultural Science, Kyushu University

• Takahashi Hideki

  RIKEN Plant Science Center Department of Biochemistry and Molecular Biology, Michigan State University

書誌事項

タイトル別名

• 5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of <i>SULFATE TRANSPORTER 1;2</i> in <i>Arabidopsis</i> roots

抄録

Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 34 (1), 51-55, 2017

  日本植物バイオテクノロジー学会

キーワード

• sulfate transporter
• SULTR1;2
• −S-inducible expression
• translation
• 5′UTR

詳細情報

• CRID: 1390282679305590016
• NII論文ID: 130005519596
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.16.1226a
• ISSN: 13476114
• ISSN: 13424580
• HANDLE: 2324/4377829
• NDL書誌ID: 028091806
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028091806
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/34/1/34_16.1226a/_pdf
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
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