Protective role of testis-specific peroxiredoxin 4 against cellular oxidative stress

• Tasaki Eisuke

  Department of Applied Bioresources Chemistry, The United Graduate School of Agriculture, Tottori University

• Matsumoto Shotaro

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Tada Hisashi

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Kurahashi Toshihiro

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Zhang Xuhong

  Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University

• Fujii Junichi

  Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University

• Utsumi Toshihiko

  Department of Applied Bioresources Chemistry, The United Graduate School of Agriculture, Tottori University Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University Graduate School of Sciences and Technology for Innovation, Yamaguchi University

• Iuchi Yoshihito

  Department of Applied Bioresources Chemistry, The United Graduate School of Agriculture, Tottori University Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University Graduate School of Sciences and Technology for Innovation, Yamaguchi University

抄録

Peroxiredoxin (PRDX), a newly discovered antioxidant enzyme, has an important role in hydrogen peroxide reduction. Among six PRDX genes (PRDX1–6) in mammals, PRDX4 gene is alternatively spliced to produce the somatic cell form (PRDX4) and the testis specific form (PRDX4t). In our previous study, PRDX4 knockout mice displayed testicular atrophy with an increase in cell death due to oxidative stress. However, the antioxidant function of PRDX4t is unknown. In this study, we demonstrate that PRDX4t plays a protective role against oxidative stress in the mammalian cell line HEK293T. The PRDX4t-EGFP plasmid was transferred into HEK293T cells; protein expression was confirmed in the cytoplasm. To determine the protective role of PRDX4t in cells, we performed image-based analysis of PRDX4t-EGFP expressed cells exposed to UV irradiation and hydrogen peroxide using fluorescent probe CellROX. Our results suggested that PRDX4t-EGFP expressed cells had reduced levels of oxidative stress compared with cells that express only EGFP. This study highlights that PRDX4t plays an important role in cellular antioxidant defense.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 60 (3), 156-161, 2017

  一般社団法人　日本酸化ストレス学会

キーワード

• antioxidant activity
• fluorescence image
• oxidative stress
• peroxiredoxin
• ROS detection

詳細情報

• CRID: 1390282679649092992
• NII論文ID: 130005632009
• DOI: 10.3164/jcbn.16-96
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://www.jstage.jst.go.jp/article/jcbn/60/3/60_16-96/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018000811
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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