Preliminary Evaluation of Glyceric Acid-producing Ability of Acidomonas methanolica NBRC104435 from Glycerol Containing Methanol

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  • Sato Shun
    Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • Kitamoto Dai
    Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • Habe Hiroshi
    Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST) Present address: Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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  • Preliminary Evaluation of Glyceric Acid-producing Ability of <i>Acidomonas methanolica</i> NBRC104435 from Glycerol Containing Methanol

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<p>Some acetic acid bacteria produce large amounts of glyceric acid (GA) from glycerol in culture broth. However, methanol, which is a major contaminant of raw glycerol derived from the biodiesel fuel industry, sharply decreases cell growth and GA production [AMB Express, 3, 20, 2013]. Thus, we evaluated the methylotrophic acetic acid bacterium Acidomonas methanolica NBRC104435 for its ability to produce GA from glycerol containing methanol. This strain accumulated GA in its culture broth when 1–3 wt% glycerol was available as a carbon source. We observed improved cell growth and GA accumulation when 1 vol% methanol was added to the 3–5 wt% glycerol medium. The maximum concentration of GA was 12.8 g/L in medium containing 3 wt% glycerol plus 1 vol% methanol. In addition, the enantiomeric excess (ee) of the GA produced was revealed to be 44%, indicating that this strain converted glycerol to d-GA with a lower enantioselectivity than other acetic acid bacteria, which had 70–99% ee.</p>

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