UPLC-MS/MS Analysis of Methotrexate in Human Plasma and Comparison with the Fluorescence Polarization Immunoassay

• MEI Shenghui

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University

• ZHU Leting

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University

• LI Xingang

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University

• WANG Jiaqing

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University

• JIANG Xueyun

  Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University

• CHEN Haiyan

  Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University

• HUO Jiping

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University

• YANG Li

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University

• LIN Song

  Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University

• ZHAO Zhigang

  Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University

抄録

Methotrexate (MTX) plasma concentration is routinely monitored to guide the dosage regimen of rescue drugs. This study aims to develop and validate an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for plasma MTX analysis, and to establish its agreement with the fluorescence polarization immunoassay (FPIA) in patients with high-dose MTX therapy. Separation was achieved by gradient elution with methanol and water (0.05% formic acid) at 40°C with a run time of 3 min. The intra- and inter-day inaccuracy and imprecision of the UPLC-MS/MS method were –4.25 to 3.1 and less than 7.63%, respectively. The IS-normalized recovery and matrix effect were 87.05 to 92.81 and 124.43 to 134.57%. The correlation coefficients between UPLC-MS/MS and FPIA were greater than 0.98. The UPLC-MS/MS method was in agreement with the FPIA at high levels of MTX (1.0 – 100 μmol/L), but not at low levels (0.01 – 1.0 μmol/L). Further studies are warranted to confirm these results.

収録刊行物

• Analytical Sciences

  Analytical Sciences 33 (6), 665-670, 2017

  社団法人 日本分析化学会

キーワード

• UPLC-MS/MS
• methotrexate
• human blood plasma
• fluorescence polarization immunoassay
• method development and validation
• method comparison
• Bland–Altman plot

詳細情報

• CRID: 1390001204260807680
• NII論文ID: 130005696791
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.33.665
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 028299129
• PubMed: 28603183
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028299129
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.33.665.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.33.665/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/33/6/33_665/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018145496
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可