Solid-Phase Extraction Spectrophotometric Determination of Total Antioxidant Capacity in Antioxidant-poor Samples by Using the Ferric-Ferrozine Method

• BERKER Kadriye Isil

  Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University

• OZYURT Dilek

  Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University

• DEMIRATA Birsen

  Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University

• APAK Resat

  Department of Chemistry, Faculty of Engineering, Istanbul University Turkish Academy of Sciences (TUBA)

抄録

Ferrozine (FZ) preferentially stabilizes Fe(II) over Fe(III) to raise the ferric reduction potential and oxidize antioxidants. The advantages of the ferric-ferrozine method over other iron-based total antioxidant capacity assays were: (i) higher molar absorptivity and enhanced sensitivity, (ii) lower interference from foreign ions, (iii) wide pH tolerance (iv) additivity of the absorbances for mixtures. Solid-phase extraction (SPE) could be combined with spectrophotometry, because the magenta-colored anionic Fe(II)-FZ complex was quantitatively sorbed on Sephadex QAE A-25 resin. The sensitivity enhancement using the resin enabled us to conduct total antioxidant capacity (TAC) measurements of antioxidant-poor samples. The apparent molar absorptivity, linear concentration range and trolox equivalent antioxidant capacities (TEAC) of certain antioxidants were found. The calibration curves (lines) of trolox, rutin, and rosmarinic acid individually and in herbal infusions—by using the method of standard additions—were parallel, confirming that the added antioxidants did not interact with herbal constituents to cause chemical deviations from Beer’s law.

収録刊行物

• Analytical Sciences

  Analytical Sciences 33 (6), 683-689, 2017

  社団法人 日本分析化学会

キーワード

• Antioxidant capacity
• solid-phase extraction
• ferric ferrozine
• spectrophotometry
• fruit juice

詳細情報

• CRID: 1390001204260809472
• NII論文ID: 130005696793
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.33.683
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 028299163
• PubMed: 28603186
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028299163
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.33.683.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.33.683/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/33/6/33_683/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018145499
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可