Fast and single-step fluorescence-based competitive bioassay microdevice using combined PDMS microchannel arrays separately immobilizing graphene oxide-analyte conjugates and fluorescently-labelled receptor proteins

• SHIRAI Akihiro

  Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

• NAKASHIMA Kaho

  Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

• SUEYOSHI Kenji

  Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

• ENDO Tatsuro

  Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

• HISAMOTO Hideaki

  Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

書誌事項

タイトル別名

• Fast and Single-step Fluorescence-based Competitive Bioassay Microdevice Combined PDMS Microchannel Arrays Separately Immobilizing Graphene Oxide–Analyte Conjugates and Fluorescently-labelled Receptor Proteins

抄録

In this study, a fast and easy-to-use capillary-type microdevice for competitive bioassay is proposed. The device is composed of polydimethylsiloxane (PDMS) microchannel arrays that separately immobilize polyethylene glycol (PEG) coating, which contained graphene oxide (GO)–analyte conjugates and fluorescently-labelled receptor proteins. The working principle of the device involved the spontaneous dissolution of the PEG coating, subsequent mixing and reaction with analyte to give fluorescence response, triggered by the capillary action-mediated introduction of a sample solution. For principle verification, a competitive biotin assay was successfully demonstrated within 20 s in a single-step operation by detecting the change in fluorescence via microscopy.

収録刊行物

• Analytical Sciences

  Analytical Sciences 33 (8), 969-972, 2017

  社団法人 日本分析化学会

キーワード

• Graphene oxide
• single-step bioassay
• fluorescence resonance energy transfer
• biotin
• microchannel array

詳細情報

• CRID: 1390282679237386240
• NII論文ID: 130005950160
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.33.969
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 028444288
• PubMed: 28794336
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028444288
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.33.969.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.33.969/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/33/8/33_969/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可