Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds

• MURAKAMI Shusuke

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

• MIYAJI Hirofumi

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

• NISHIDA Erika

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

• KAWAMOTO Kohei

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

• MIYATA Saori

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

• TAKITA Hiroko

  Support Section for Education and Research, Hokkaido University Graduate School of Dental Medicine

• AKASAKA Tsukasa

  Department of Dental Materials and Engineering, Hokkaido University Graduate School of Dental Medicine

• FUGETSU Bunshi

  Nano-Agri Lab, Policy Alternatives Research Institute, The University of Tokyo

• IWANAGA Toshihiko

  Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine

• HONGO Hiromi

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• AMIZUKA Norio

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• SUGAYA Tsutomu

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

• KAWANAMI Masamitsu

  Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine

抄録

Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of β-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.

収録刊行物

• Dental Materials Journal

  Dental Materials Journal 36 (5), 573-583, 2017

  日本歯科理工学会

キーワード

• Biomaterial
• Cell ingrowth
• Nano-dispersion
• Bone tissue engineering

詳細情報

• CRID: 1390282679694124288
• NII論文ID: 130006100273
• NII書誌ID: AA10443149
• DOI: 10.4012/dmj.2016-295
• ISSN: 18811361
• ISSN: 02874547
• HANDLE: 2115/68057
• PubMed: 28450672
• Web Site: https://www.jstage.jst.go.jp/article/dmj/36/5/36_2016-295/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018363126
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可