Efficient and scheduled production of pseudopregnant female mice for embryo transfer by estrous cycle synchronization
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- HASEGAWA Ayumi
- RIKEN BioResource Center, Ibaraki 305-0074, Japan
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- MOCHIDA Keiji
- RIKEN BioResource Center, Ibaraki 305-0074, Japan
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- OGONUKI Narumi
- RIKEN BioResource Center, Ibaraki 305-0074, Japan
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- HIROSE Michiko
- RIKEN BioResource Center, Ibaraki 305-0074, Japan
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- TOMISHIMA Toshiko
- RIKEN BioResource Center, Ibaraki 305-0074, Japan
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- INOUE Kimiko
- RIKEN BioResource Center, Ibaraki 305-0074, Japan Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan
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- OGURA Atsuo
- RIKEN BioResource Center, Ibaraki 305-0074, Japan Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan The Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113-0033, Japan
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<p> In embryo transfer experiments in mice, pseudopregnant females as recipients are prepared by sterile mating with vasectomized males. Because only females at the proestrus stage accept males, such females are selected from a stock of animals based on the appearance of their external genital tract. Therefore, the efficiency of preparing pseudopregnant females largely depends on the size of female colonies and the skill of the operators who select females for sterile mating. In this study, we examined whether the efficiency of preparing pseudopregnant females could be improved by applying an estrous cycle synchronization method by progesterone (P4) pretreatment, which significantly enhances the superovulation outcome in mice. We confirmed that after two daily injections of P4 (designated Days 1 and 2) in randomly selected females, the estrous cycles of most females (about 85%) were synchronized at metestrus on Day 3. When P4-treated females were paired with vasectomized males for 4 days (Days 4–8), a vaginal plug was found in 63% (20/32) of the females on Day 7. After the transfer of vitrified-warmed embryos into their oviducts, 52% (73/140) of the embryos successfully developed into offspring, the rate being comparable to that of the conventional embryo transfer procedure. Similarly, 77% (24/31) of females became pregnant by fertile mating with intact males for 3 days, which allowed the scheduled preparation of foster mothers. Thus, our estrous cycle synchronization method may omit the conventional experience-based process of visually observing the vagina to choose females for embryo transfer. Furthermore, it is expected that the size of female stocks for recipients can be reduced to less than 20%, which could be a great advantage for facilities/laboratories undertaking mouse-assisted reproductive technology.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 63 (6), 539-545, 2017
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337958656
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- NII論文ID
- 130006250262
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 028718592
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- PubMed
- 28824024
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可