FISH with Padlock Probes Can Efficiently Reveal the Genomic Position of Low or Single-Copy DNA Sequences
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- Matsunaga Sachihiro
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- Matsunaga Tomoko M.
- Research Institute for Science and Technology, Tokyo University of Science
抄録
<p>A padlock probe is a circularized oligonucleotide containing complementary sequences of target regions. Padlock probes combined with rolling-circle amplification enable fluorescence in situ hybridization (FISH) to reproducibly detect the genomic position of low or single-copy DNA sequences. The FISH with padlock probes revealed that a plant gene cluster of tandemly arrayed three paralogues responsible for photosynthesis rapidly moved from the interior subnuclear region to the peripheral subnuclear region in response to light. Using a pair of peptide nucleic acids as a double-strand opener for the target region, the efficiency of FISH with padlock probes at the detection of a single-copy genomic region was much improved. FISH with padlock probes will give us reliable data of dynamics, arrangement and position of a specific single locus in both the nucleus and the condensed chromosomes.</p>
収録刊行物
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- CYTOLOGIA
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CYTOLOGIA 82 (4), 337-339, 2017
公益財団法人 日本メンデル協会
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詳細情報 詳細情報について
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- CRID
- 1390282679087586816
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- NII論文ID
- 130006250977
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- ISSN
- 13487019
- 00114545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可