High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein

• Lili Geng

  State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

• Deng Xu

  State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Microalgae Laboratory, School of Resource & Environmental Engineering, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology

• Minhong Zhang

  Institute of Animal Science, Chinese Academy of Agricultural Sciences

• Changlong Shu

  State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

• Jinghai Feng

  Institute of Animal Science, Chinese Academy of Agricultural Sciences

• Fuping Song

  State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

• Fan Lu

  Microalgae Laboratory, School of Resource & Environmental Engineering, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology

• Jie Zhang

  State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

書誌事項

タイトル別名

• High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric <i>Bacillus thuringiensis</i> Protein

抄録

Many types of Bacillus thuringiensis (Bt)-crops are being grown worldwide, triggering concerns about their potential impact on humans and livestock. To ensure better yield and food safety in China, an attempt has been made to develop Bt-rice targeting a broad range of insects. We aimed to investigate whether feeding genetically modified rice expressing the Bt chimeric Cry1Ac/Cry1Ab protein has any effects on the intestinal microbiota of broilers. Broilers were fed either Bt-rice or its unmodified isogenic parent line for 42 days, and total DNA was isolated from cecum contents for high-throughput sequencing of the 16S rRNA gene. In total, 1,241,005 reads, assigned to 12 phyla, 31 families, and 48 genera were generated. No significant differences were observed in the relative abundance of organisms identified among the major phyla, families, and genera, except for two less abundant families, Thermoanaerobacteraceae and Peptostreptococcaceae, and two less abundant genera, Anaerotruncus and Gelria. The results were in agreement with those from culture-based analysis and Biolog EcoPlates. These results illustrate that feeding Bt-rice has no adverse effects on the broiler intestinal microbiota and provide sufficient support for the food safety of Bt-rice.

収録刊行物

• The Journal of Poultry Science

  The Journal of Poultry Science 55 (1), 10-16, 2018

  日本家禽学会

キーワード

• broilers
• Bt-rice
• high-throughput sequencing-based analysis
• intestinal microbiota

詳細情報

• CRID: 1390282680182955008
• NII論文ID: 130006320803
• NII書誌ID: AA11564513
• DOI: 10.2141/jpsa.0170029
• ISSN: 13490486
• ISSN: 13467395
• NDL書誌ID: 028779668
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028779668
• Web Site: https://www.jstage.jst.go.jp/article/jpsa/55/1/55_0170029/_pdf
• 本文言語コード: en
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