Algal Genes Encoding Enzymes for Photosynthesis and Hydrocarbon Biosynthesis as Candidates for Genetic Engineering

DOI Web Site 被引用文献3件 参考文献51件
  • Uchida Hidenobu
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Kato Ko
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • Suzuki Kensaku
    Tohoku Agricultural Research Center, National Agriculture and Food Research Organization
  • Yokota Akiho
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • Kawano Shigeyuki
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
  • Matsunaga Shigeki
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Okada Shigeru
    Graduate School of Agricultural and Life Sciences, The University of Tokyo

抄録

<p>In order to increase the biomass of photosynthetic organisms, upregulation of net CO2 fixation in daytime appears to be a promising approach. This can be attained by improving the turnover of chloroplast enzymes such as ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) or fructose bisphosphate phosphatase (FBPase). In a previous report, the genes for the large and small subunits of cyanobacterial Rubisco were coexpressed in tobacco with either with cyanobacterial chaperone for Rubisco or the carboxysomal CO2 concentrating mechanism (CCM) gene. This resulted in the generation of faster carboxylation of Rubisco and a similar gene expression approach may be adopted into algal hosts in the future. Expression of the cyanobacterial FBPase gene with protein targeting to the chloroplast in Euglena gracilis was reported to increase the wax ester content effectively. Though stable transformation of the oil producing alga Botryococcus braunii race B, is yet to be achieved, genes for key regulatory enzymes in 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, such as 1-deoxy-D-xylulose 5-phosphate synthase (DXS) or 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), might be possible targets for genetic modification in this alga. Challenging problems in the transformation of the microalgae, including C. reinhardtii, or those of the macroalgae are discussed herein for the design of transformation aiming at higher yield of biofuel production.</p>

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (51)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ