<b>Syntaxin 6 is Involved in the Maintenance of Secretory Granules in Parotid Acinar Cells </b>

• Yokoyama Megumi

  Department of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Katsumata-Kato Osamu

  Department of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Fujita-Yoshigaki Junko

  Department of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

抄録

Secretory granules (SGs) of salivary glands are considered to be generated as an immature granule and to mature by condensing their contents. Syntaxin 6 and vesicle associated membrane protein 4 (VAMP4) are detected in the membrane fractions of immature but not mature SGs, suggesting that syntaxin 6 and VAMP4 are transported from SGs to other organelles during the process of granule maturation. To study the role of syntaxin 6 in the biogenesis of granules, we transfected syntaxin 6 fused with enhanced green fluorescent protein(EGFP)into primary cultured parotid acinar cells. The EGFPsyntaxin 6 signal overlapped with that of an early endosome marker, early endosome antigen 1(EEA1), which was detected using immunofluorescence. The localization of EGFP-fused VAMP4 was also similar to that of EEA1, suggesting that syntaxin 6 and VAMP4 are transported from immature SGs to early endosomes. Transfection of a syntaxin 6 mutant that lacks a SNARE-binding domain caused a decrease in the number of SGs although the localization of the mutant was unchanged from that of the wild type syntaxin 6. These results suggest that syntaxin 6 has an important role in the maintenance of SGs in parotid acinar cells.

収録刊行物

• ＩＪＯＭＳ

  ＩＪＯＭＳ 15 (3-4), 67-73, 2017

  日本大学松戸歯学部 口腔科学研究所

キーワード

• salivary glands
• secretory granules
• secretory grasyntaxin 6
• VAMP4
• early endosomes

詳細情報

• CRID: 1390282680190471296
• NII論文ID: 130006894939
• DOI: 10.5466/ijoms.15.67
• ISSN: 21854254
• ISSN: 13479733
• Web Site: https://www.jstage.jst.go.jp/article/ijoms/15/3-4/15_67/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018000287
• 本文言語コード: en
• データソース種別:
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