Flower Color Modification by Suppression of Chalcone Synthase Gene in Gentian.
-
- Nishihara Masahiro
- Iwate Biotechnology Research Center
-
- Nakatsuka Takashi
- Iwate Biotechnology Research Center
-
- Mishiba Keiichiro
- Iwate Biotechnology Research Center
-
- Kikuchi Akiko
- Iwate Biotechnology Research Center
-
- Yamamura Saburo
- Iwate Biotechnology Research Center
Bibliographic Information
- Other Title
-
- カルコン合成酵素遺伝子抑制によるリンドウの花色変異体作出
Abstract
We have already obtained transgenic gentians that turned to be white flower color by transformation with antisense CHS. Here we report the characteristics of them. Because the transformants had a bar gene in the genome, they were expected to have herbicide-resistance. Two lines of transformants were subjected to herbicide including 18% bialaphos and tested for herbicide tolerance. Application with 200-fold dilution of herbicide showed that transformants were resistant to this herbicide, while untransformed plants died within one month. Furthermore, their pollen fertility, plant height and flowering date were investigated. Both fertility by aceto-carmine staining and plant height are not significantly different from those of untransformed plants. Flowering date was a few earlier in transgenics but it may be due to pot cultivation in greenhouse. Now we are attempting to suppress CHS using inverted repeat vector. Some transformants show white to faint blue-colored flower in culture vessels.
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2003 (0), 538-538, 2003
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390282680606215936
-
- NII Article ID
- 130006989961
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed