Characterization of enf1 Mutant, which is Defective in Shoot Apical Meristem and Adaxial-abaxial Axis, by Transcriptome and Metabolome Analyses

• Toyokura Koichi

  Dept. of Sci., Kyoto Univ.

• Kusano Miyako

  PSC, RIKEN

• Watanabe Keiro

  Dept. of Sci., Kyoto Univ.

• Tameshige Toshiaki

  Dept. of Sci., Kyoto Univ. NIBB

• Tatematsu Kiyoshi

  NIBB

• Tsugeki Ryuji

  Dept. of Sci., Kyoto Univ.

• Matsumoto Noritaka

  Dept. of Sci., Kyoto Univ.

• Saito Kazuki

  PSC, RIKEN

• Okada Kiyotaka

  NIBB

Bibliographic Information

Other Title

• 茎頂分裂組織および向背軸に異常を示す変異体enf1のトランスクリプトーム・メタボローム解析

Abstract

Development of shoot apical meristem (SAM) and the adaxial-abaxial (ad-ab) axis of leaf primordial is important for shoot development. It is known that SAM and ad-ab axis mutually control their development. We isolated enf1 mutant, which is defective in both SAM and the ad-ab axis. The expression patterns of the key transcription factors, FIL and WUS were altered in enf1 mutant, suggesting that ENF1 regulate SAM and ad-ab axis via transcriptional regulation of the transcription factors. Transcriptome analysis also revealed that ENF1 regulated several transcription factors. ENF1 gene encoded an enzyme in the primary metabolism, and metabolome analysis showed a drastic change of metabolites in SAM of the mutant. It is suggested that the metabolic condition of cells is responsible to gene expression profile. We will discuss the relationship among metabolite, transcription, and morphogenesis.

Journal

• Plant and Cell Physiology Supplement

  Plant and Cell Physiology Supplement 2009 (0), 0004-0004, 2009

  The Japanese Society of Plant Physiologists

Details

• CRID: 1390001205631184256
• NII Article ID: 130006992255
• DOI: 10.14841/jspp.2009.0.0004.0
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed