腫瘍マーカー開発のためのがんのプロテオーム研究
書誌事項
- タイトル別名
-
- Cancer proteomics for biomarker development
抄録
Cancer is the leading cause of disease death in Japan. Despite recent progress of early diagnostic modalities and therapeutic management, the long-term survival of cancer patients has been still poor and the extensive efforts to improve the prognosis have been devoted to cancer research. To identify the proteins associated with various clinical states of cancer and to utilize such proteins as diagnostic and prognostic biomarkers, we established the proteome analysis system based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and bioinformatics approach. In our system, the quantitative protein expression profiles are generated by two-dimensional difference gel electrophoresis (2D-DIGE), in which protein samples are labeled with fluorescence dyes prior to 2D-PAGE. As high sensitive fluorescent dyes enable high-throughput laser microdissection, 2D-DIGE can achieve accurate clinical proteomic study. Multivariate algorithms and statistical-learning methods determine unique protein expression patterns corresponding to clinical features and mass spectrometric study identifies the proteins involved in the patterns. Proteomic study using cancer cell lines and clinical specimens of various types of cancer revealed that our proteome analysis system can identify the protein expression patters corresponding to 1) original organ and histology, 2) properties of metastasis and invasion, or 3) histological subtype of malignancies.
収録刊行物
-
- 日本プロテオーム学会大会要旨集
-
日本プロテオーム学会大会要旨集 2004 (0), 81-81, 2004
日本プロテオーム学会（日本ヒトプロテオーム機構）
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282680610079872
-
- NII論文ID
- 130006995419
-
- データソース種別
-
- JaLC
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可
