In-capillary enzymatic reaction electrophoresis for the sensitive analysis of glycans from glycoproteins including biopharmaceuticals

• Suzuki Shigeo

  Faculty of Pharmaceutical Sciences, Kindai University

Bibliographic Information

Other Title

• インキャピラリー酵素反応電気泳動による抗体医薬品等の糖タンパク質糖鎖の高感度解析

Abstract

Here we introduce in-capillary lectin-binding and exoglycosidase-digestion methods using a plug-plug kinetic mode capillary electrophoresis for the analysis of glycoprotein-derived oligosaccharides derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS). The application of various specificities of lectins indicated characteristic migration profiles of the oligosaccharides. Reactions with neuraminidase and α/β-galactosidase digestions were completed by separation voltage, but hexosaminidase and fucosidase/mannosidase require more drastic conditions such as zero potential amplification and low mixing voltage application, respectively. The methods were applied to the detection of high-mannose type oligosaccharides in a mixture of glycans and NeuGc and α-Gal epitopes in IgG pharmaceuticals.

Journal

• Electrophoresis Letters

  Electrophoresis Letters 62 (1), 23-26, 2018

  Japanese Electrophoresis Society

Keywords

• plug-plug kinetic capillary electrophoresis
• lectins
• exoglycosidases

Details

• CRID: 1390845712973228416
• NII Article ID: 130007393787
• DOI: 10.2198/electroph.62.23
• ISSN: 21892636
• ISSN: 21892628
• Web Site: https://www.jstage.jst.go.jp/article/electroph/62/1/62_23/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019103019
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed