Isolation and Functional Characterization of New Terpene Synthase Genes from Traditional Edible Plants
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- Hattan Jun-ichiro
- Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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- Shindo Kazutoshi
- Department of Food and Nutrition, Japan Women’s University
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- Sasaki Tetsuya
- Industrial Research Institute of Ishikawa
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- Misawa Norihiko
- Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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<p>Terpene synthase (TPS) genes were isolated and functionally characterized from three traditional edible plants, Acanthopanax sciadophylloides (“Koshiabura”) and Acanthopanax sieboldianus (“Himeukogi”), belonging to the family Araliaceae, and Curcuma zedoaria (zedoary, “Gajutsu”), belonging to the family Zingiberaceae. These plants emit characteristic fragrances and are used for traditional foods and folk medicines. From their fragrant tissues, i.e., sprouts of Araliaceae plants and developing rhizomes of zedoary, total RNAs were extracted and reverse transcribed. The resultant cDNAs were used for degenerate PCR followed by rapid amplification of cDNA ends. From the contig sequences obtained, full-length Tps genes were amplified by PCR with newly synthesized primer sets. The isolated full-length genes were introduced into engineered Escherichia coli cells, which can utilize acetoacetate to synthesize farnesyl diphosphate, the substrate for TPSs, through the mevalonate pathway. TPS products synthesized in the transformed E. coli cells were analysed by gas chromatography-mass spectrometry, nuclear magnetic resonance, and optical rotation. Consequently, the isolated Tps genes were found to encode β-caryophyllene synthase, germacrene D synthase, linalool/(3S)-(+)-nerolidol synthase, β-eudesmol synthase, and germacrene B synthase. These results lead us to expect that some of the effective ingredients in folk medicines are volatile terpenes and that intake of traditional foods including these edible plants would have some positive effects on our health.</p>
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 67 (10), 1235-1246, 2018
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282763058748160
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- NII論文ID
- 130007497979
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 029238670
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- PubMed
- 30305556
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可