Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells

DOI Web Site 参考文献29件
  • Liu Ji-Luan
    Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University
  • Yang Li-Cong
    Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University
  • Zhu Xiao-Juan
    Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University
  • Wang We-Jun
    Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University
  • Zheng Guo-Dong
    Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University

抄録

<p>The aim of the study was to investigate the combinational effect of Pine needle polysaccharide (PNP) and Kudzu flavonoids (KF) on cell differentiation and fat metabolism in 3T3-L1 cells. 3T3-L1 cells were treated with various concentrations of PNP or/and KF. Cell viability, Oil red O staining, intracellular TG, glycerol in culture medium, levels of cytokines and expression of transcription factors genes were evaluated. PNP and KF combination significantly suppressed cells differentiation and fat accumulation by inhibiting the mRNA expression of PPARγ2 (peroxisome proliferator activated receptor-γ2), C/EBPα (CCAAT/enhancer binding proteins-α) and SREBP-1c (sterol regulatory element binding protein-1c) in 3T3-L1 cells. In addition, the gene expression and secretion of leptin and IL-6 were inhibited, adiponectin was significantly increased, secretion of TNF-α was inhibited by PNP and KF combination. Taken together, PNP and KF combination may exert anti-adipogenic effects through regulation of lipid metabolism gene expression of the transcription factors and cytokines. The findings suggested that PNP and KF combination may be explored as a potentially promising food additive for prevention of obesity.</p>

収録刊行物

参考文献 (29)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ