Morphological analysis for neuronal pathway from the hindbrain ependymocytes to the hypothalamic kisspeptin neurons
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- DEURA Chikaya
- Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan
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- MINABE Shiori
- Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan
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- IKEGAMI Kana
- Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan
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- INOUE Naoko
- Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan
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- UENOYAMA Yoshihisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan
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- MAEDA Kei-ichiro
- Department of Veterinary Medical Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- TSUKAMURA Hiroko
- Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan
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<p> Hindbrain ependymocytes are postulated to have a glucose-sensing role in regulating gonadal functions. Previous studies have suggested that malnutrition-induced suppression of gonadotropin secretion is mediated by noradrenergic inputs from the A2 region in the solitary tract nucleus to the paraventricular nucleus (PVN), and by corticotropin-releasing hormone (CRH) release in the hypothalamus. However, no morphological evidence to indicate the neural pathway from the hindbrain ependymocytes to hypothalamic kisspeptin neurons, a center for reproductive function in mammals, currently exists. The present study aimed to examine the existence of a neuronal pathway from the hindbrain ependymocytes to kisspeptin neurons in the arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV). To determine this, wheat-germ agglutinin (WGA), a trans-synaptic tracer, was injected into the fourth ventricle (4V) in heterozygous Kiss1-tandem dimer Tomato (tdTomato) rats, where kisspeptin neurons were visualized by tdTomato fluorescence. 48 h after the WGA injection, brain sections were taken from the forebrain, midbrain and hindbrain and subjected to double immunohistochemistry for WGA and dopamine β-hydroxylase (DBH) or CRH. WGA immunoreactivities were found in vimentin-immunopositive ependymocytes of the 4V and the central canal (CC), but not in the third ventricle. The WGA immunoreactivities were detected in some tdTomato-expressing cells in the ARC and AVPV, DBH-immunopositive cells in the A1–A7 noradrenergic nuclei, and CRH-immunopositive cells in the PVN. These results suggest that the hindbrain ependymocytes have neuronal connections with the kisspeptin neurons, most probably via hindbrain noradrenergic and CRH neurons to relay low energetic signals for regulation of reproduction.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 65 (2), 129-137, 2019
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390564238083031296
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- NII論文ID
- 130007630928
- 40021868983
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 029637662
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- PubMed
- 30662010
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可