Efficient in vitro embryo production using in vivo-matured oocytes from superstimulated Japanese Black cows

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  • EGASHIRA Junki
    Faculty of Agriculture, Saga University, Saga 840-8502, Japan The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan Saga Prefectural Livestock Experiment Station, Saga 849-2305, Japan
  • IHARA Yusuke
    Faculty of Agriculture, Saga University, Saga 840-8502, Japan
  • KHATUN Hafiza
    Faculty of Agriculture, Saga University, Saga 840-8502, Japan The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • WADA Yasuhiko
    Faculty of Agriculture, Saga University, Saga 840-8502, Japan The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan
  • KONNO Toshihiro
    The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan
  • TATEMOTO Hideki
    The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan
  • YAMANAKA Ken-ichi
    Faculty of Agriculture, Saga University, Saga 840-8502, Japan The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan

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  • Efficient <i>in vitro</i> embryo production using <i>in vivo</i>-matured oocytes from superstimulated Japanese Black cows

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<p> We examined whether the use of in vivo-matured oocytes, collected by ovum pick-up (OPU) from superstimulated Japanese Black cows, can improve the productivity and quality of in vitro produced embryos. The cows were superstimulated by treatment with progesterone, GnRH, FSH and prostaglandin F2α according to a standardized protocol. The resulting in vivo-matured oocytes were collected by OPU and used subsequently for the other experiments. The immature oocytes from cows in the non-stimulated group were collected by OPU and then subjected to maturation in vitro. We found that the rate of normally distributed cortical granules of the matured oocyte cytoplasm in the superstimulated group was significantly higher than that in the non-stimulated group. The normal cleavage rate (i.e., production of embryos with two equal blastomeres without fragmentation) and freezable blastocyst rate were significantly higher in the superstimulated group than in the non-stimulated group. Among the transferable blastocysts, the ratio of embryos from normal cleavage was also significantly higher in the superstimulated group than in the non-stimulated group. For in vivo-matured oocytes, it was observed that the pregnancy rates were significantly higher when normally cleaved embryos were used for transfer. Taken together, these results suggest that high-quality embryos with respect to developmental kinetics can be efficiently produced with the use of in vivo-matured oocytes collected by OPU from superstimulated Japanese Black cows.</p>

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