薬剤性アレルギー様反応に関連するヒトMRGPRX2のイヌオルソログ同定に関する研究

DOI

書誌事項

タイトル別名
  • Identification of the dog orthologue of human MAS-related G protein coupled receptor X2 (MRGPRX2) essential for drug-induced pseudo-allergic reactions

抄録

<p>MAS-related G protein coupled receptor-X2 (MRGPRX2) is a Gi- or Gq-coupled receptor expressed in human mast cells and associated with drug-induced pseudo-allergic reactions. Dogs are highly susceptible to drug-induced anaphylactoid reactions caused by various agents including fluoroquinolones; however, the distribution and physiological function of canine MRGPR family genes, including MRGPRX2, remain largely unknown. In the present study, we clarified the distribution of dog MRGPR family genes (D, F, G, and X2) by quantitative PCR and in situ hybridization. We also investigated the stimulatory effects of compound 48/80 and several fluoroquinolones (ciprofloxacin [CPFX], gatifloxacin [GFLX], levofloxacin [LVFX], and pazufloxacin [PZFX]) on HEK293 cells transiently transfected with dog MRGPR family genes to identify the physiological function. Dog MRGPRX2 and MRGPRG were distributed in a limited number of tissues, including the skin (from the eyelid, abdomen, and cheek), whereas MRGPRD and MRGPRF were extensively expressed in almost all tissues examined. Histochemical and in situ hybridization analyses revealed that MRGPRX2 was expressed in dog connective tissue-type mast cells in the skin. Intracellular Ca2+ mobilization assay revealed that HEK293 cells expressing dog MRGPRX2 or human MRGPRX2, but not dog MRGPRD, MRGPRF and MRGPRG, responded to histamine releasing agents. Our results suggest that dog MRGPRX2 is the functional orthologue of human MRGPRX2 and plays an essential role in drug-induced anaphylactoid reactions in dogs.</p>

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390848647545025536
  • NII論文ID
    130007898359
  • DOI
    10.14869/toxpt.47.1.0_p-60e
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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