Development of New PCR and Quantitative PCR Protocols for the Detection of Plecoglossus altivelis Poxvirus-like Virus in Atypical Cellular Gill Disease of Ayu

• Koyama Tomoki

  Tokyo University of Marine Science and Technology

• Komatsu Daiki

  Tokyo University of Marine Science and Technology

• Uchino Tsubasa

  Tokyo University of Marine Science and Technology Aquaculture Research Department, Fisheries Technology Institute, Japan Fisheries Research and Education Agency

• Midorikawa Yuki

  Tokyo University of Marine Science and Technology

• Kato Goshi

  Tokyo University of Marine Science and Technology

• Ishikawa Takanori

  Tochigi Prefectural Fisheries Experimental Station

• Nishimura Tomohiro

  Tochigi Prefectural Fisheries Experimental Station

• Takeda Korenori

  Tochigi Prefectural Fisheries Experimental Station

• Fukuda Hideo

  Tokyo University of Marine Science and Technology

• Wada Shinpei

  Nippon Veterinary and Life Science University

• Sano Motohiko

  Tokyo University of Marine Science and Technology

書誌事項

タイトル別名

• Development of New PCR and Quantitative PCR Protocols for the Detection of <i>Plecoglossus altivelis</i> Poxvirus-like Virus in Atypical Cellular Gill Disease of Ayu

抄録

Plecoglossus altivelis poxvirus-like virus (PaPV) is the presumptive causative agent of atypical cellular gill disease (ACGD), which has caused significant losses in aquaculture of ayu P. altivelis in Japan. ​In this study, we investigated a new PCR protocol and a quantitative PCR (qPCR) protocol with primers designed from the vaccinia virus A16L homologous gene sequence of PaPV. ​The new PCR showed a positive reaction for affected fish in all eight ACGD cases tested and no cross-reaction with carp edema virus, and was more sensitive than a PCR with BOKE30 primers. ​The qPCR quantified around 10⁸–10⁹ virus DNA copies/mg in the diseased fish gills.

収録刊行物

• 魚病研究

  魚病研究 55 (3), 84-87, 2020-09-15

  日本魚病学会

キーワード

• <i>Plecoglossus altivelis</i> poxvirus-like virus
• PCR
• qPCR
• atypical cellular gill disease
• ayu

詳細情報

• CRID: 1390849376466032128
• NII論文ID: 130007928676
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.55.84
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 030704619
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030704619
• Web Site: https://www.jstage.jst.go.jp/article/jsfp/55/3/55_84/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
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