Cellular expression and subcellular localization of diacylglycerol kinase γ in rat brain
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- HOZUMI Yasukazu
- Department of Cell Biology and Morphology, Akita University Graduate School of Medicine Department of Anatomy and Cell Biology, Yamagata University School of Medicine
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- NAKANO Tomoyuki
- Department of Anatomy and Cell Biology, Yamagata University School of Medicine
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- GOTO Kaoru
- Department of Anatomy and Cell Biology, Yamagata University School of Medicine
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<p>Gq protein-coupled receptors lead to activation of phospholipase C, which triggers phosphoinositide signaling. Diacylglycerol (DG) is one of the phosphoinositide metabolites and serves as a second messenger. Diacylglycerol kinase (DGK) phosphorylates DG to produce another second messenger phosphatidic acid. Of the DGK family, DGKγ is predominantly expressed in the brain at the mRNA level. Recent studies have shown the expression of DGKγ in vascular endothelial cells and adrenal medullary cells at the protein level, although its detailed cellular expression pattern and subcellular localization in the brain remain to be determined. In the present study, we addressed this point using specific DGKγ antibody. DGKγ was expressed in both projection neurons and interneurons in the cerebral cortex, hippocampal formation, and cerebellum. In cerebellar Purkinje cells, DGKγ was distributed to the soma and dendrites. Fractionation study revealed that DGKγ was enriched in the internal membranes containing the endoplasmic reticulum and Golgi complex. In immunoelectron microscopy, DGKγ was localized throughout the smooth endoplasmic reticulum system. These findings suggest that DGKγ shows unique cellular expression pattern in the brain and distinct subcellular localization different from other DGK isozymes.</p>
収録刊行物
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- Biomedical Research
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Biomedical Research 42 (1), 33-42, 2021-02-10
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