Control of cell adhesion and detachment using temperature and thermoresponsive copolymer grafted culture surfaces

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<jats:title>Abstract</jats:title><jats:p>The hydrophobic monomer, <jats:italic>n</jats:italic>‐butyl methacrylate (BMA) has been incorporated into thermoresponsive poly(<jats:italic>N</jats:italic>‐isopropylacrylamide) (PIPAAm) to lower PIPAAm phase transition temperatures necessary for systematically regulating cell adhesion on and detachment from culture dishes at controlled temperatures. Poly(IPAAm‐<jats:italic>co</jats:italic>‐BMA)‐grafted dishes were prepared by electron beam irradiation methods, systematically changing BMA content in the feed. Copolymer‐grafted surfaces decreased grafted polymer transition temperatures with increasing BMA content as shown by water wettabilities compared to homopolymer PIPAAm‐grafted surfaces. Bovine endothelial cells readily adhered and proliferated on copolymer‐grafted surfaces above collapse temperature at 37°C, finally reaching confluence. Cell sheet detachment behavior from copolymer‐grafted surfaces depended on the culture temperature and BMA content. In conclusion, cell attachment/detachment can be controlled to an arbitrary temperature by varying the content of hydrophobic monomer incorporated into PIPAAm grafted to culture surfaces. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 70–78, 2004</jats:p>

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