<i>Drosophila</i>MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems

• Qing-Xin Liu

  Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies, Mishima,Shizuoka-ken 411-8540, Japan

• Marek Jindra

  Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies, Mishima,Shizuoka-ken 411-8540, Japan

• Hitoshi Ueda

  Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies, Mishima,Shizuoka-ken 411-8540, Japan

• Yasushi Hiromi

  Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies, Mishima,Shizuoka-ken 411-8540, Japan

• Susumu Hirose

  Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies, Mishima,Shizuoka-ken 411-8540, Japan

抄録

During gene activation, the effect of binding of transcription factors to cis-acting DNA sequences is transmitted to RNA polymerase by means of co-activators. Although co-activators contribute to the efficiency of transcription, their developmental roles are poorly understood. We usedDrosophila to conduct molecular and genetic dissection of an evolutionarily conserved but unique co-activator, Multiprotein Bridging Factor 1 (MBF1), in a multicellular organism. Through immunoprecipitation, MBF1 was found to form a ternary complex including MBF1, TATA-binding protein (TBP) and the bZIP protein Tracheae Defective (TDF)/Apontic. We have isolated aDrosophila mutant that lacks the mbf1 gene in which no stable association between TBP and TDF is detectable, and transcription of a TDF-dependent reporter gene is reduced by 80%. Although the null mutants ofmbf1 are viable, tdf becomes haploinsufficient inmbf1-deficient background, causing severe lesions in tracheae and the central nervous system, similar to those resulting from a complete loss oftdf function. These data demonstrate a crucial role of MBF1 in the development of tracheae and central nervous system.

収録刊行物

• Development

  Development 130 (4), 719-728, 2003-02-15

  The Company of Biologists

キーワード

• Developmental Biology
• Molecular Biology

詳細情報

• CRID: 1360011145699541888
• NII論文ID: 30002331983
• DOI: 10.1242/dev.00297
• ISSN: 14779129
• ISSN: 09501991
• Web Site: http://journals.biologists.com/dev/article-pdf/130/4/719/1499191/719.pdf
• データソース種別:
  • Crossref
  • CiNii Articles