Loss of Tbx4 blocks hindlimb development and affects vascularization and fusion of the allantois

• L. A. Naiche

  Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, 701 W. 168th Street, New York, NY 10032,USA

• Virginia E. Papaioannou

  Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, 701 W. 168th Street, New York, NY 10032,USA

抄録

Tbx4 is a member of the T-box family of transcription factor genes, which have been shown to play important roles in development. We have ablated Tbx4 function using targeted mutagenesis in the mouse. Embryos homozygous for the null allele fail to undergo chorioallantoic fusion and die by 10.5 days post coitus. The allantoises of Tbx4-mutant embryos are stunted, apoptotic and display abnormal differentiation. Endothelial cells within mutant allantoises do not undergo vascular remodeling. Heterozygous embryos show a mild, transient growth defect in the allantois. Induction of a hindlimb field occurs normally in Tbx4mutants and initial patterning of the hindlimb bud appears normal. However,hindlimb buds from Tbx4 mutants fail to develop either in vivo or in vitro and do not maintain Fgf10 expression in the mesenchyme. The expression of another, closely-linked, T-box gene, Tbx2, is reduced in both the hindlimb and the allantois of Tbx4-mutant embryos prior to the development of overt morphological abnormalities, which suggests that Tbx4 regulates Tbx2 in these tissues.

収録刊行物

• Development

  Development 130 (12), 2681-2693, 2003-06-15

  The Company of Biologists

キーワード

• Developmental Biology
• Molecular Biology

詳細情報

• CRID: 1361418520689353216
• NII論文ID: 30002332176
• DOI: 10.1242/dev.00504
• ISSN: 14779129
• ISSN: 09501991
• Web Site: http://journals.biologists.com/dev/article-pdf/130/12/2681/1142953/2681.pdf
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