The potential to induce glial differentiation is conserved between<i>Drosophila</i>and mammalian glial cells missing genes

DOI PDF 被引用文献4件
  • Yasuno Iwasaki
    Division of Molecular Neurobiology, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, Japan
  • Toshihiko Hosoya
    Department of Developmental Genetics, National Institute of Genetics, Mishima,Shizuoka 411-8540, Japan
  • Hirohide Takebayashi
    Division of Molecular Neurobiology, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, Japan
  • Yasuhiro Ogawa
    Division of Molecular Neurobiology, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, Japan
  • Yoshiki Hotta
    Department of Developmental Genetics, National Institute of Genetics, Mishima,Shizuoka 411-8540, Japan
  • Kazuhiro Ikenaka
    Division of Molecular Neurobiology, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, Japan

抄録

<jats:p>Drosophila glial cells missing (gcm) is a key gene that determines the fate of stem cells within the nervous system. Two mouse gcm homologs have been identified, but their function in the nervous system remains to be elucidated. To investigate their function, we constructed retroviral vectors harboring Drosophila gcm and two mouse Gcm genes. Expression of these genes appeared to influence fibroblast features. In particular, mouse Gcm1 induced the expression of astrocyte-specific Ca2+-binding protein, S100β, in those cells. Introduction of the mouse Gcm1 gene in cultured cells from embryonic brains resulted in the induction of an astrocyte lineage. This effect was also observed by in utero injection of retrovirus harboring mouse Gcm1 into the embryonic brain. However, cultures from mouse Gcm1-deficient mouse brains did not exhibit significant reductions in the number of astrocytes. Furthermore,in situ hybridization analysis of mouse Gcm1 mRNA revealed distinct patterns of expression in comparison with other well-known glial markers. The mammalian homolog of Drosophila gcm, mouse Gcm1, exhibits the potential to induce gliogenesis, but may function in the generation of a minor subpopulation of glial cells.</jats:p>

収録刊行物

  • Development

    Development 130 (24), 6027-6035, 2003-12-15

    The Company of Biologists

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