Differentiation‐inducing factor of <i>D. discoideum</i> raises intracellular calcium concentration and suppresses cell growth in rat pancreatic AR42J cells

• Yuzuru Kubohara

• Yutaka Saito

• Kazuhiko Tatemoto

抄録

DIF (differentiation‐inducing factor) is a putative morphogen that induces stalk cell differentiation in the lower eukaryote, Dictyostelium discoideum . In this study, we have examined the effects of DIF on growth and the intracellular calcium concentration ([Ca 2+ ] i ) in rat pancreatic acinar AR42J cells. Growth of AR42J cells was inhibited when DIF was present in the media, and approximately 50% growth inhibition was attained with 20 μM DIF. DIF was also found to raise [Ca 2+ ] i in a dose‐dependent manner (1–40 μM), both in the presence and absence of extracellular Ca 2+ . These results suggest that DIF elicits both calcium influx from the extracellular space and calcium release from intracellular pool(s), thereby inhibiting cell growth in AR42J.

収録刊行物

• FEBS Letters

  FEBS Letters 359 (2-3), 119-122, 1995-02-13

  Wiley

キーワード

• Cell Biology
• Genetics
• Molecular Biology
• Biochemistry
• Structural Biology
• Biophysics

詳細情報

• CRID: 1361418519237995520
• NII論文ID: 30002583309
• DOI: 10.1016/0014-5793(95)00022-2
• ISSN: 18733468
• ISSN: 00145793
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2895%2900022-2
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(95)00022-2
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2895%2900022-2
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