Effects of EGF and TGF-α on Invasion and Proteinase Expression of Uterine Cervical Adenocarcinoma OMC-4 Cells

• Masatsugu Ueda

• Hideji Fujii

• Keiko Yoshizawa

• Yoshito Terai

• Koji Kumagai

• Ken Ueki

• Minoru Ueki

抄録

Uterine cervical adenocarcinoma typically is an aggressive neoplasm with a propensity for early invasion and dissemination; however, the regulatory mechanism of invasive activity of cervical adenocarcinoma cells has not been fully understood. In this study, biological effects of epidermal growth factor (EGF) and transforming growth factor (TGF)-α on invasion and proteinase expression of human cervical adenocarcinoma OMC-4 cells were investigated. Tumor cell migration along a gradient of substratum-bound fibronectin and invasion into the reconstituted basement membrane were stimulated by 0.1–10 nM EGF and TGF-α in a concentration-dependent manner. Their effects on tumor cell migration were also confirmed by wound assay. The zymography of tumor-conditioned medium showed that the treatment of OMC-4 cells with EGF and TGF-α resulted in the increase of matrix metalloproteinase (MMP)-2 and urokinase-type plasminogen activator (uPA). Matrilysin (MMP-7), also secreted by OMC-4 cells, was not affected by these growth factors. These results suggest that EGF and TGF-α act as positive regulators on the invasion of cervical adenocarcinoma cells, which may be associated with their stimulatory effects on tumor cell motility and the induction of type IV collagenase and uPA secreted by tumor cells.

収録刊行物

• Invasion and Metastasis

  Invasion and Metastasis 18 (4), 176-183, 1998

  S. Karger AG

キーワード

• Cancer Research

詳細情報

• CRID: 1360574095549691904
• NII論文ID: 30006246720
• DOI: 10.1159/000024510
• ISSN: 14230119
• ISSN: 02511789
• Web Site: https://www.karger.com/Article/Pdf/24510
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  • CiNii Articles