Isolation and partial characterization of the sperm motility initiation factor from eggs of the pacific herring, <i>Clupea pallasi</i>

• Muralidharan C. Pillai

• Tammy S. Shields

• Ryuzo Yanagimachi

• Gary N. Cherr

抄録

Previously, we have demonstrated that sperm from the Pacific herring, Clupea pallasi , remain immotile upon spawning into the surrounding medium and initiate motility only upon contact with the micropyle area of the chorion (Yanagimachi et al., '92, Dev. Growth Differ., 34:447–461). In the present study we have purified a chorion‐bound sperm motility initiating factor (SMIF) and have found it to be a 105 kDa glycosylated polypeptide. The isolated factor was highly bioactive, stimulating prolonged motility in >90% of the cells in vitro. The 105 kDa glycoprotein showed affinity for Con A on western blots. Furthermore, in intact eggs, Con A was localized to only the micropyle area, indicating that SMIF is restricted to this region of the chorion. © 1993 Wiley‐Liss, Inc.

収録刊行物

• Journal of Experimental Zoology

  Journal of Experimental Zoology 265 (3), 336-342, 1993-03

  Wiley

キーワード

• Animal Science and Zoology
• General Medicine

詳細情報

• CRID: 1363951793555851264
• NII論文ID: 30007076890
• NII書誌ID: AA0025201X
• DOI: 10.1002/jez.1402650316
• ISSN: 1097010X
• ISSN: 0022104X
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1402650316
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1402650316
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